The NCAA unveiled its largest-ever Division II women’s lacrosse championship bracket on Sunday evening, identifying 24 teams that will begin the chase this week for the national championship.
The expanded bracket features six teams selected from each of the four geographic regions and included, for the first time, automatic qualifiers that won league championships. Seven teams, including five AQ squads, earned their first-ever NCAA tournament berth: Ashland, Dominican, Embry-Riddle, Frostburg State, Molloy, Rockhurst, and Slippery Rock.
The top two seeds from each region received first-round byes and are serving as host sites for NCAA first- and second-round games. Those teams are:
Atlantic Region: No. 1 West Chester and No. 2 Slippery Rock
East Region: No. 1 Adelphi and No. 2 Pace
Midwest Region: No. 1 Maryville and No. 2 UIndy
South Region: No. 1 Tampa and No. 2 Florida Southern
Winners of second-round games on May 10 will advance to the national quarterfinals on May 17. The four quarterfinal winners (regional champions) will then be re-seeded for the national semifinals on May 22 in Salem, Va. The championship game is May 24.
Here’s a closer look at what the NCAA selection committee rolled out Sunday evening.
Best First-Round Game
There are several potentially entertaining games among Thursday’s eight opening-round contests, but Wingate v. Limestone in the Atlantic is our choice. And with all due respect to Wingate, which earned its second NCAA berth in program history, most of the intrigue in this matchup lies with the Saints.
Last week, Limestone University announced that after 180 years, it was closing its doors due to financial troubles. The school held its final commencement ceremony Saturday, roughly at the same time that the Saints were upending Wingate, 16-7, to win the South Atlantic Conference championship and secure the league’s AQ for the NCAA tournament. Now, five days later, these league rivals meet again for the third time this year.
But while the Limestone players will wear their traditional blue and gold uniforms at least once more, the school that they represent does not technically exist anymore.
All that aside, Limestone has one last chance to chase the glory that used to be theirs, as long before the emergence of the Sunshine State Conference, the Saints were the torch bearers for women’s lacrosse in the South Region. While this is their first (and last) NCAA appearance since 2021, this is Limestone’s 12th appearance over the past 21 years. The Saints were national runners-up in 2011 and 2013, and a deep run in this year’s tournament would be a wonderful farewell celebration of their program.
First- or Second-Round Upset Alert
It's always tricky to peg an upset, but one of the factors that can help is familiarity. Is the lower seed comfortable facing the higher seed? Based on that criterion, we’re tabbing East Stroudsburg, No. 3 in the Atlantic Region, to defeat No. 2 Slippery Rock in the second round. The presumption is that ESU will slip past Frostburg in round one to earn the date against The Rock. This would be the third meeting of the year between the PSAC rivals, and the second in a span of five days. Slippery Rock won both prior matchups, including a 12-11 overtime victory in Friday’s league semifinal. The Rock earned its first NCAA berth this season, while ESU is back in the tournament for the eighth straight year and aiming for its seventh straight trip to the regional finals.
Toughest Region
The South Region, which includes four teams from the Sunshine State Conference, is undoubtably the most competitive, as it has been throughout the 2025 season. In fact, it’s the only region in which five of the six teams boast a strength of schedule rating of over .600.
Defending champion and No. 1 seed Tampa (17-1) is the favorite but will likely have to beat either No. 2 seed Florida Southern (17-2) for a third time or get past No. 3 seed Embry-Riddle, which handed Tampa its only loss of the year. No. 4 seed Lynn and No. 5 Flagler are the highest-ranked four and five seeds in the bracket.